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Upright microscopes are used for phase contrast or wide field fluorescence microscopy of samples or living cells that are pressed within a cover slip and a slide. The microscopy of tissue sections or fixed cells is the additional application. For live-cell imaging, particularly with dipping objective lenses, these microscopes can be used. This type of method is commonly used for large samples such as animals and tissues. Upright microscopes have longer working distances, resulting in lower resolution and weaker fluorescence signals. Upright microscopes are not suitable for use with high numerical aperture objectives directly on cover slip bottoms, to which cells adhere.
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In February 2021, Nikon announced the release of the ECLIPSE Si upright microscope. Features include an ergonomic design, automatic shut-off mode and intelligent time-saving features to reduce physical strain from prolonged microscope use.
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